United Kingdom (UK)
Recombinant monoclonal antibody to Capsid protein VP25. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma PL-2 scFv.
UniProt Accession Number of Target Protein: Q82446
Alternative Name(s) of Target: Capsid polyprotein VP90; Capsid polyprotein VP70; Capsid protein VP34; Capsid protein VP27; Human astrovirus-2
Immunogen: The original antibody was raised by immunizing a BALB/c mouse with Human astrovirus-2.
Specificity: This antibody is specific for Capsid protein VP25 from Human astrovirus-2.
Application Notes: The structure of the original antibody was determined using x-ray crystallography. This antibody was used for a western blot on HAstV-2 capsid protein (Bogdanoff et al., 2016; PMID:27213181). The original antibody was used for an ELISA on purified Spike or GFP-Spike from HAstV-2 (Bogdanoff et al., 2017; PMID:27807234, Bogdanoff et al., 2018; PMID:29070688), and on purified Human astrovirus-2 (Sanchez-Fauquier et al., 1994; PMID:7514320). Further this antibody was used for surface plasmon resonance on Spike2 from HAstV-2. This antibody has shown that it is able to block the GFP-Spike2 binding to Caco-2 cells (Bogdanoff et al., 2017; PMID:27807234). This antibody was used for immunofluorescence on Human astrovirus-2 infected LLCMK2. This antibody was used for radioimmunoprecipitation on virus samples (Sanchez-Fauquier et al., 1994; PMID:7514320). This antibody was used for a neutralization assay by preincubating human astrovirus-2 with this antibody and then adding C2Bbe1 cells (Bogdanoff et al., 2018; PMID:29070688).
Antibody first published in:
Sanchez-Fauquier et al. Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization Virology. 1994 Jun;201(2):312-20. PMID:7514320
Note on publication:
The original paper identified several unique proteins in human astrovirus serotype 2 by using newly generated antibodies.