United Kingdom (UK)
Recombinant monoclonal antibody to Carbonic anhydrase. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody cAbCA05.
UniProt Accession Number of Target Protein: P00921
Alternative Name(s) of Target: cAb-CA05; carbonic anhydrases; carbonate dehydratases; CA
Immunogen: The original antibody was generated by immunizing a dromedary with bovine erythrocytecarbonic anhydrase. A phage display library was constructed and the antibody was selected by panning.
Specificity: The antibody is specific for carbonic anhydrase.
Application Notes: The crystal structures of a the VHH antibody both as free antibody (Decanniere et al., 2000; PMID: 10864500 and Desmyter et al. 2000; PMID: 11342547) and in complex with its antigen (Desmyter et al., 2000; PMID: 11342547) were determined. The VHH antibody recognized the bovine erythrocyte carbonic anhydrase with an affinity of 72 nM (Kd) (Desmyter et al., 2000; PMID: 11342547). The antibody did not inhibit the enzymatic activity of the antigen (Desmyter et al., 2000; PMID: 11342547 and Lauwereys et al., 1998; PMID: 9649422).
Antibody first published in:
Lauwereys et al. Potent enzyme inhibitors derived from dromedary heavy-chain antibodies. EMBO J. 1998 Jul 1;17(13):3512-20. PMID:9649422
Note on publication:
The original paper describes the generation and characterization of the antibody