United Kingdom (UK)
Recombinant monoclonal antibody to CCL20. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AB1.
UniProt Accession Number of Target Protein: O89093
Alternative Name(s) of Target: C-C motif chemokine 20; Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20
Specificity: The antibody is specific for murine CCL20. The antibody does not recognise human CCL20.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. Capture ELISA was performed and the 1:1 binding kinetics and affinities were measured by surface plasmon resonance (Kd= 2.3 nM). CCR6 Chinese hamster ovary (CHO)-K1 β-arrestin G protein–coupled receptor functional assay was performed to measure the inhibition of the antibody (IC50= 30.7).
Antibody first published in:
Cannon et al. Experimentally guided computational antibody affinity maturation with de novo docking, modelling and rational design PLoS Comput Biol. 2019 May; 15(5): e1006980. PMID:31042706
Note on publication:
The original paper describes the generation and computational affinity maturation of the antibody.