United Kingdom (UK)
Recombinant monoclonal antibody to CD11a. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma NKIL16.
UniProt Accession Number of Target Protein: P20701
Alternative Name(s) of Target: NKI-L16; Integrin alpha-L; ITGAL; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain
Immunogen: The mouse version of this antibody was raised by immunizing a BALB/c mosue with human cytotoxic T-lymphocytes.
Specificity: This antibody is specific for CD11a. CD11a is a receptor for ICAM1, ICAM2, ICAM3, ICAM4, F11R, and the secreted form of ubiquitin-like protein ISG15. CD11a is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. It contributes to natural killer cell cytotoxicity.
Application Notes: To show that the enhanced adhesiveness of CD4+ T cells is induced by CD3 cross-linking, an adhesion assay was performed on human PMBCs using the mouse version of this antibody (Mazerolles et al, 1991; pmid:1826886). An adhesion assay was performed on human T and B cells in the presence of the mouse version of this antibody to study the kinetics of T-cell adhesion (Mazerolles et al, 1991; pmid:1679053).
Antibody first published in:
Mazerolles et al. Regulation of LFA-1-mediated T cell adhesion by CD4 Eur J Immunol. 1991 Apr;21(4):887-94. PMID:1826886
Note on publication:
To show that the enhanced adhesiveness of CD4+ T cells induced by CD3 cross-linking using plastic-bound anti-CD3 antibody can also be inhibited by several CD4 ligands. i.e. anti-CD4 antibodies, the gp160 env protein of human immunodeficiency virus, as well as by putative CD4 ligands, i.e. synthetic peptides analogous to the gp160-binding site to CD4 (positions 418-434 and 449-464) and a 12-mer synthetic peptide (DR-12) analogous to positions 35-46 of HLA class II beta subunit and including the highly conserved Arg-Phe-Asp-Ser (RFDS) sequence.
Immunofluorescence staining of Jurkat cells with anti-CD11a antibody NKIL16 (Ab02892). Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of NKIL16 (Ab02892-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab02892-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.