United Kingdom (UK)
Recombinant monoclonal antibody to CD14. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 18D11.
UniProt Accession Number of Target Protein: P08571
Alternative Name(s) of Target: Monocyte antigen; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
Immunogen: The original antibody was raised by immunizing a mouse with human CD14.
Specificity: This antibody is specific for human CD14.
Application Notes: Western blot was performed using this antibody on proteins (US20170107294). This antibody was used for flow cytometery on fresh human or porcine whole blood (US20170107294) (Lau et al, 2013; pmid:24062486). ELISA was performed on C1q and human FcgRs using this antibody (US20170107294). Blocking experiments were performed to investigate the role of CD14 using this antibody (Lotz et al, 2004; pmid:14673018).
Antibody first published in:
Lotz et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 2004 Mar;75(3):467-77. PMID:14673018
Note on publication:
The interaction of highly purified human PMN with endotoxin-free LTA from Staphylococcus aureus was investigated.