- Ab00123-8.1 Anti-CD16/CD32 [2.4G2]
- Rat IgG2b
- Purified, Vivopure
- In Stock
- Ab00123-24.1 Anti-CD16/CD32 [2.4G2]
- Goat IgG
- In Stock
- Ab00123-23.0 Anti-CD16/CD32 [2.4G2]
- Rabbit IgG
- In Stock
Recombinant monoclonal antibody to CD16/CD32. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2.4G2.
UniProt Accession Number of Target Protein: P08508; P08101
Alternative Name(s) of Target: Fcγ III/II Receptor; Low affinity immunoglobulin gamma Fc region receptor III; IgG Fc receptor III; Fc-gamma RIII; FcRIII; CD16; Low affinity immunoglobulin gamma Fc region receptor II; Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII IgG Fc rec
Immunogen: Mouse BALB/c Macrophage J774 Cell Line.
Specificity: This antibody binds to an epitope on the extracellular domains of the mouse FcγIII and FcγII, thereby inhibiting their ability to bind antibodies via their Fc region. receptors. Also binding the FcγI receptor (CD64) via its Fc domain.
Application Notes: This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies made by Absolute Antibody that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors.
Antibody first published in:
Unkeless JC. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979 Sep 19;150(3):580-96. PMID:90108
Note on publication: Describes the generation of rat monoclonal antibodies directed against the mouse FcγIII and FcγII receptors.
Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (Ab00123-8.1, clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.
Anti-CD16/32 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/32 antibody Ab00123-8.1 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD16/32-positive population is apparent.