Anti-CD18 [1B4]

Name: Anti-CD18 [1B4]
SKU: Ab01201
Availability: InStock

Description:

Recombinant monoclonal antibody to CD18. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1B4.

Clone Name:

1B4

Published Application(s):

IP, RIA, ELISA, FC, IF

Published Species Reactivity:

Human, Rhesus Monkey

Products based on this clone:

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Ab01201-2.0 Anti-CD18 [1B4]
Human, Rhesus Monkey
Mouse IgG2a
Purified
In Stock

Ab01201-2.3 Anti-CD18 [1B4]
Human, Rhesus Monkey
Mouse IgG2a
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01201-23.0 Anti-CD18 [1B4]
Human, Rhesus Monkey
Rabbit IgG
Purified
In Stock

Ab01201-10.0 Anti-CD18 [1B4]
Human, Rhesus Monkey
Human IgG1
Purified
Ships in 4-5 weeks

Ab01201-10.3 Anti-CD18 [1B4]
Human, Rhesus Monkey
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P05107

Alternative Name(s) of Target: Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; ITGB2; MFI7; IB4; m1B4; mIB4
Immunogen: Blood was obtained from 4 helathy donors and the mononuclear cells harvested and grown. A BALB/c mouse was injected with 10^6 monocytes three times at monthly intervals, and four times with 5x10^6 monocytes oon each of the four days before fusion. The spleen cells were then fused to P3U1 myeloma cells to generate hybridomas and RIA was used to identify lines of interest.

Specificity: Specific to the beta chain of the leukocyte integrin beta-2 family. This is found in LFA-1, Mac-1 and p150.95 among others. Binds an epitope distinct from the C3bi binding site. Has been shown to recognise both the human and Rhesus monkey antigen.

Application Notes: The main application 1B4 has been proposed for is its anti-inflammatory activity. It has been shown to work as a Fab fragment prepared by papain digestion (Wright et al, 1983). Its binding to antigen was confirmed by RIA (Van Voorhis et al, 1983). 1B4 has been shown to work in Rhesus monkeys, and has reduced immunogenicity once humanized. 1B4 is also compatible with immunofluorescence which has been used to demonstrate that the fine specificity of the combining sites was not altered by humanization (Singer et al, 1993). It has been shown to prevent polymorphonuclear (PMN)-dpepndent pathology in animal models of hemorrhagic shock, dermal inflammation, reperfusion injury, IBD and meningitis (Vedder et al, 1988, Vedder et al, 1990, Mileski et al, 1990, Carlos et al, 1990, Tuomanen et al, 1989, Arfors et al, 1987, Wallace et al, 1991, Wallace et al, 1992). The glycosylation patterns of 1B4 have also been studied in detail using NMR, ES-MS, HPAEC-PAD mapping and glycosyl composition analysis (Yu Ip et al, 1994).

Antibody first published in: Wright et al Identification of the C3bi receptor of human monocytes and macrophages by using monoclonal antibodies. Proc Natl Acad Sci U S A. 1983 Sep; 80(18): 5699–5703 PMID:6225125
Note on publication: Describes the generation of this IB4 and others, analysis of their binding by competitive binding experiments to identify different/common epitopes, immunoprecipitation and ELISAs to confirm a common antigen.

Immunofluorescence on human peripheral blood monocytes using Ab01201-23.0. Click on image for more details.

Immunofluorescence staining of human peripheral blood monocytes using anti-CD18 antibody (Ab01201) 1B4 Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of 1B4 (Ab01201-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01201-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.

Flow cytometry on human peripheral blood leukocytes using ab01201-23.0. Click on image for more details.

Flow-cytometry using the anti-CD18 antibody 1B4 (Ab01201). Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 1B4 (Ab01201-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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