United Kingdom (UK)
Recombinant monoclonal antibody to CD19. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 6D5.
UniProt Accession Number of Target Protein: P15391
Alternative Name(s) of Target: Leu12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19 T-cell surface antigen Leu-12; CD19; B4
Immunogen: This antibody was raised by immunising rats with mouse CD19-expressing K562 human erythroleukemia cells.
Specificity: This antibody recognises mouse CD19, which is a transmembrane glycoprotein that regulates B lymphocyte development, activation, and differentiation. This clone 6D5 recognizes the same epitope as the published clone 1D3.
Application Notes: 6D5 antibody has been extensively used to identify B cells in various experimental arrangements. It can be efficiently utilised in the localisation of B cells in various tissues. For instance, one group stained murine spleen tissue cryosections to visualise lymphomas in azathioprine-treated mice (Chalastanis et al., 2010). This antibody has also been widely used in flow cytometry. One group utilised in the flow cytometric identification of B cells in a study investigating the relation of microglia depletion and MPTP neurotoxicity (Yang et al., 2018).
Antibody first published in:
Krop et al. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan;26(1):238-42. PMID:8566073
Note on publication:
This article describes the method of generation of antibodies like 6D5.
Flow cytometry using the Anti-CD19 antibody 6D5 (Ab01489). Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 6D5 (Ab01489-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.