Anti-CD19 [HD37]

Description:

Recombinant monoclonal antibody to CD19. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HD37.

Clone Name:

HD37

Published Application(s):

IP, Block, FC, IF, IHC

Published Species Reactivity:

Human

Products based on this clone:

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Ab00214-1.1 Anti-CD19 [HD37]
Human
Mouse IgG1
Purified
In Stock

Ab00214-23.0 Anti-CD19 [HD37]
Human
Rabbit IgG
Purified
In Stock

Ab00214-1.4 Anti-CD19 [HD37]
Human
Mouse IgG1
Fc Silent™
Purified
In Stock

UniProt Accession Number of Target Protein: P15391

Alternative Name(s) of Target: Leu12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19 T-cell surface antigen Leu-12; CD19
Immunogen: Cells from a hairy cell leukemia patient.

Specificity: Recognises human CD19.

Application Notes: This antibody binds to CD19 and is able to inhibit B-cell activation (Pezutto 1987).

Antibody first published in: Pezzutto et al. HD37 Monoclonal Antibody: A Useful Reagent for Further Characterization of “Non-T, Non-B” Lymphoid Malignancies Leukocyte Typing II, pp 391-402, 1986, ISBN 978-1-4612-4848-4, DOI 10.1007/978-1-4612-4848-4_33 PMID:
Note on publication: Describes the generation and characterization of this antibody.

Flow-cytometry on human lymphocytes using Ab00214. Click on image for more details.

Flow-cytometry using anti-CD19 antibody HD37 (Ab00214) Human lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of HD37 (Ab00214-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.