Anti-CD1d [1B1]

Name: Anti-CD1d [1B1]
SKU: Ab02718
Availability: InStock

Description:

Recombinant monoclonal antibody to CD1d. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1B1.

Clone Name:

1B1

Published Application(s):

therapeutic, ELISA, FC, IF, IHC

Published Species Reactivity:

Mouse

Products based on this clone:

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Ab02718-23.0 Anti-CD1d [1B1]
Mouse
Rabbit IgG
Purified
In Stock

Ab02718-8.1 Anti-CD1d [1B1]
Mouse
Rat IgG2b
Purified
Ships in 4-5 weeks

Ab02718-8.4 Anti-CD1d [1B1]
Mouse
Rat IgG2b
Fc Silent™
Purified
Ships in 4-5 weeks

Ab02718-3.0 Anti-CD1d [1B1]
Mouse
Mouse IgG2b
Purified
Ships in 4-5 weeks

Ab02718-3.3 Anti-CD1d [1B1]
Mouse
Mouse IgG2b
Fc Silent™
Purified
Ships in 4-5 weeks

Ab02718-2.0 Anti-CD1d [1B1]
Mouse
Mouse IgG2a
Purified
In Stock

UniProt Accession Number of Target Protein: P11609

Alternative Name(s) of Target: Antigen-presenting glycoprotein CD1d1; Cd1d1; CD1d.1
Immunogen: The original version of this antibody was raised by immunizing Lewis rats using mCD1-transfected RMA-S cells.

Specificity: This antibody is specific for CD1d. Its binding is blocked by antibody clone 3D11. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.

Application Notes: In an attempt to further the understanding of CD1 proteins, flow cytometry was preformed on mouse spleen cells using the rat version of this antibody. Further, mice infected with L. monocytogenes were treated with the rat version of this antibody. Finally, mice spleen cells were stained with the mouse version of this antibody (Szalay et al, 1999; pmid:10358132). To investigate the tissue distribution of murine CD1 in mice, immunohistochemistry was preformed on thymus, small intestine, and spleen tissue of mice using the rat version of this antibody. Further, flow cytometry was preformed on epithelial cells and IEL using the rat version of this antibody (Brassay et al, 1997; pmid:9233616). In order to measure mCD1-restricted T cell responses to glycolipids, the rat version of this antibody was added after which cytokine levels were assessed by ELISA (Burdin et al, 1998; pmid:9759842). To assess the role of CD1 in the pathogenesis of lupus the rat version of this antibody was used for immunofluorescent staining and flow cytometry on single cell suspensions of mouse spleen cells or mouse bone marrow. The mouse version of this antibody was further tested in vivo on mice with lupus. Their lifespan showed an increase (Zeng et al, 2000; pmid:10799851). To study the expression of CD1 on B cell subsets from normal and b2m2/2 mice, single-cell suspensions of lymphoid cells from C57BL/6 and BALB/c mice were used for immunofluorescence using the mouse version of this antibody (Amano et al, 1998; pmid:9712035). To eludicate the location of expression of CD1 flow cytometry was preformed on thymocytes from wild-type, b2m-deficient, and TAP-1-deficient C57BL/6 mice (Roark et al, 1998; pmid:9531266).

Antibody first published in: Brossay et al. Mouse CD1 is mainly expressed on hemopoietic-derived cells J Immunol. 1997 Aug 1;159(3):1216-24. PMID:9233616
Note on publication: New mAbs to mCD1 to investigate its tissue distribution were generated.

Immunofluorescence staining of mouse splenocytes using Ab02718-23.0. Click on image for more details.

Immunofluorescence staining of mouse splenocytes with anti-CD1d antibody 1B1 (Ab02718). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 1B1 (Ab02718-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab02718-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

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