Anti-CD20 [B9E9]

Name: Anti-CD20 [B9E9]
SKU: Ab01139
Availability: InStock

Description:

Recombinant monoclonal antibody to CD20. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma B9E9.

Clone Name:

B9E9

Published Application(s):

radioimmunotherapy, WB

Published Species Reactivity:

Human

Products based on this clone:

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Ab01139-2.0 Anti-CD20 [B9E9]
Human
Mouse IgG2a
Purified
In Stock

Ab01139-2.3 Anti-CD20 [B9E9]
Human
Mouse IgG2a
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01139-23.0 Anti-CD20 [B9E9]
Human
Rabbit IgG
Purified
In Stock

Ab01139-10.0 Anti-CD20 [B9E9]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab01139-10.3 Anti-CD20 [B9E9]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01139-13.12 Anti-CD20 [B9E9]
Human
Human IgG4-S228P
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P11836

Alternative Name(s) of Target: MS4A1; B-lymphocyte antigen CD20;MS4A1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; E9PPL6; P19437
Immunogen: This antibody was raised against human CD20.

Specificity: This antibody is specific for CD20, which is expressed by normal and malignant B cells. It does not cross-react with murine CD20.

Application Notes: This antibody has been used in western blot detection of CD20 in human plasma, revealing elevated CD20 levels in plasma from chronic lymphocytic leukemia patients (Manshouri et al, 2003). An scFv derived from this antibody fused to streptavidin has been investigated as a method of pretargeted radioimmunotherapy for lymphomas (Schultz et al, 2000), and has been demonstrated to be non-toxic in a phase I trial of non-Hodgkin lymphoma patients (Forero et al, 2004). Additionally, measles viruses engineered to express this scFv are able to enter CD20+ target cells through interaction with CD20 antigen, with clinical relevance for lymphoma therapy (Bucheit et al, 2003).

Antibody first published in: Schultz et al. A Tetravalent Single-chain Antibody-Streptavidin Fusion Protein for Pretargeted Lymphoma Therapy Cancer Res. 60 (23), 6663-6669 (2000) PMID:11118050
Note on publication: Describes the generation of an scFv from this antibody.

IF staining of Daudi cells using Ab01139-23.0 Click on image for more details.

Immunofluorescence staining of Daudi cells with anti-CD20 (Ab01139) B9E9 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells stained with the chimeric rabbit IgG version of B9E9 (Ab01139-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01139-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (Ab00102-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.