United Kingdom (UK)
Recombinant monoclonal antibody to CD32. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MDE-8.
UniProt Accession Number of Target Protein: P12318
Alternative Name(s) of Target: CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a
Immunogen: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund’s adjuvant.
Specificity: This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a on peripheral blood monocytes, polymorphonuclear cells and platelets.
Application Notes: The binding ability of MDE-8 to FcgammaRIIa was confirmed using flow cytometry. MDE-8 binds to FcgammaRIIa on cell lines, peripheral bloodmonocytes, PMN, platelets and blocked ligand binding via this receptor. It can also effectively blocked FcγRIIa ligand-binding via its F(ab')2 fragment. Overnight incubation of monocytes with F(ab')2 fragments of MDE-8 leads to a 60% decrease in cell surface expression of FcγRIIa. MDE-8 whole antibody induced a concomitant 30% decrease of FcγRI on THP-1cells and monocytes (PMID: 15982355). MDE-8 was reoported to induce Fc-mediated hypothermia and severe thrombocytopenia in FCGR2A mice (PMID: 26396093).
Antibody first published in:
van Royen-Kerkhof et al. A novel human CD32 mAb blocks experimental immune haemolytic anaemia in FcgammaRIIA transgenic mice. Br J Haematol. (2005); 130(1):130-7. PMID:15982355
Note on publication:
Describes the generation and characterization of the antibody.
Flow cytometry using the Anti-CD32 antibody MDE-8 (Ab02072). Paraformaldehyde fixed U937 cells were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of MDE-8 (Ab02072-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.