- Ab03965-23.0 Anti-CD32b [NOV0281]
- Rabbit IgG
- Ships in 5-6 weeks
Recombinant monoclonal antibody to CD32b. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody NOV0281.
UniProt Accession Number of Target Protein: P31994
Alternative Name(s) of Target: CD32; Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; human FCGR2B
Immunogen: The original antibody was isolated from a human combinatorial antibody library by panning against biotinylated human CD32b.
Specificity: The antibody specifically binds to CD32b. The antibody mainly recognizes epitope I (i.e. the Fc binding domain area). The antibody does not cross reacts with human CD32a-R.
Application Notes: The specificity of the antibody for CD32b was determined by ELISA analysis. The antibody was able to bind to CD32b expressed on human B cells by flow cytometry analysis (binding affinity 5.4 nM). The binding the antibody to the BJAB cell line was evaluated by flow cytometry (binding affinity = 2.4 nM). The antibody bound to CHO cells expressing huCD32b by flow cytometry analysis. The human IgG1 format of the antibody was evaluated for its activity in a primary NK cell based antibody-dependent cell-mediated cytotoxicity (ADCC) assay. The antibody demonstrated concentration dependent specific cell lysis of both cancer cell lines evaluated (Jeko-1 and Karpas422 Cancer Cell) (US20170198040A1).
Antibody first published in: PMID: