Anti-CD36 [185-1G2 (B467)]

Description:

Recombinant monoclonal antibody to CD36. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 185-1G2 (B467).

Clone Name:

185-1G2 (B467)

Published Application(s):

Blocking, IP, FC, IF, IHC

Published Species Reactivity:

Human

6

Products based on this clone:

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Ab01539-21.0 Anti-CD36 [185-1G2 (B467)]
Human
Mouse IgM
Purified
In Stock

Ab01539-1.1 Anti-CD36 [185-1G2 (B467)]
Human
Mouse IgG1
Purified
In Stock

Ab01539-23.0 Anti-CD36 [185-1G2 (B467)]
Human
Rabbit IgG
Purified
In Stock

Ab01539-15.0 Anti-CD36 [185-1G2 (B467)]
Human
Human IgM
Purified
Ships in 4-5 weeks

Ab01539-10.0 Anti-CD36 [185-1G2 (B467)]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab01539-10.3 Anti-CD36 [185-1G2 (B467)]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

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UniProt Accession Number of Target Protein: Q8WTV0

Alternative Name(s) of Target: SRB1; Scavenger receptor class B member 1; CD36 and LIMPII analogous 1; CLA-1; CD36 antigen-like 1; Collagen type I receptor, thrombospondin receptor-like 1; SR-BI
Immunogen: This antibody was raised by immunizing mice with stimulated human leukocytes.

Specificity: This antibody is specific against CD36. The antibody recognises an epitope between amino acids 155 - 183. CD36 is a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. It acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry as well as mediates uptake of M. fortuitum, E. coli and S. aureus.

Application Notes: This antibody is recommended for the detection and analysis of CD36 via various methods. For instance, it was used to stain ovarian cancer tissue samples via immunohistochemistry, demonstrating that CD36 was expressed in the cytoplasm of cancer cells while stromal cells were negative (Têtu et al., 2008). Furthermore, 185-1G2 antibody was shown to precipitate CD36 from a platelate lysate sample (Miao et al., 2001). Finally, this antibody has also blocking properties against CD36 and has been widely used to inhibit this receptor (Short et al., 2005).

Antibody first published in: Miao et al. CD36 associates with CD9 and integrins on human blood platelets. Blood. 2001 Mar 15;97(6):1689-96. PMID:11238109
Note on publication: The article describes the use of the 185-1G2 antibody in immunoprecipitations.

Binding Curve

Western blot on human peripheral blood monocyte cell lysate using Ab01539-23.0. Click on image for more details.

Binding Curve

Western Blot using anti-CD36 antibody 185-1G2 (B467) (Ab01539) Human peripheral blood monocyte cell lysate (35µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 185-1G2 (B467) (Ab01539-23.0) at 0.03 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Binding Curve

IF staining of HepG2 cells using Ab01539-23.0. Click on image for more details.

Binding Curve

Immunofluorescence staining of fixed HepG2 cells with anti-CD36 antibody 185-1G2 (B467) (Ab01539) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 185-1G2 (B467) (Ab01539-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01539-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.