United Kingdom (UK)
Recombinant monoclonal antibody to CD3e. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 33H1.
UniProt Accession Number of Target Protein: P07766
Alternative Name(s) of Target: T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3-ε; CGMCC NO 21980; SP7
Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant human CD3 antigen (amino acids 23-126).
Specificity: This antibody binds human CD3e. It is part of TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. It also plays a role in signal transduction during T cell development and in correct T-cell development.
Application Notes: This binding specificity of this antibody for human CD3e was checked using ELISA. The affinity constant of the original IgG2a antibody was determined to be 1.92×10^9 M^(-1). This antibody can recognize human CD3e recombinant protein in an immunoblot assay. This antibody is similar to commercially available anti-CD3 antibody SP7 in immunohistochemical staining of T cell lymphomas. This antibody can also be used for the immunohistochemical staining of normal tissues like brain, heart, cerebellum, esophageal, adrenal, stomach, ovary, small intestine, pancreatic, colorectal, paratony gland, liver, pituitary, salivary gland, testicular, kidney, thyroid, prostate, breast, uterus, spleen , Bladder, tonsil, skeletal muscle, thymus (young children), skin, bone marrow, peripheral nerve, lung and melodder cells (CN111363043).
Antibody first published in: PMID:
Flow cytometry using anti-CD3e antibody 33H1 (Ab03155). Jurkat cells were stained with the anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 33H1 (Ab03155-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of Jurkat cells with anti-CD3e antibody 33H1 (Ab03155). Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 33H1 (Ab03155-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03155-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.