Anti-CD4 [3F11]

Description:

Recombinant monoclonal antibody to CD4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 3F11.

Clone Name:

3F11

Published Application(s):

Blocking, FACS, ELISA

Published Species Reactivity:

Human

UniProt Accession Number of Target Protein: P01730

Alternative Name(s) of Target: CD4mut; CD4 molecule; OKT4D; IMD79; 03F11; CD4#8; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

Specificity: This antibody is specific for human CD4

Application Notes: The binding of the original version of this antibody (VHH) to CD4 was characterized in an ELISA. In a FACS binding assay, this antibody exhibited an EC₅₀ of 0.7 nM, 1.0 nM, and 0.76 nM for MOLM-13, THP-1, and T cells, respectively, and in an HIV-1 neutralization it exhibited an IC₅₀ of 29.3 nM for PMBCs + NL4.3. This antibody was used to construct two bi-specific nanobodies with clone 281F12 (Ab04369 Anti-CXCR4), one where it was in the 1st position and one where it was in the 2nd position. Alone, it bound CXCR4⁺/CD4⁺ MOLM-13 cells with a binding EC₅₀ of 0.6 nM and CXCR4⁺/CD4^low Jurkat E6-1 cells with a binding EC₅₀ of 61 nM. Its bi-specific constructs bound the CXCR4⁺/CD4⁺ MOLM-13 and CXCR4⁺/CD4^low Jurkat E6-1 cells with a binding EC₅₀ of 1.1 and 11 nM when it was in the 2nd position and 0.7 and 1.1 nM when it was in the 1st position. Furthermore, its bi-specific constructs exhibited a chemotaxis IC₅₀ of 0.59 and 110 nM on CXCR4⁺/CD4⁺ MOLM-13 and CXCR4⁺/CD4^low Jurkat E6-1 cells when it was in the 2nd position, and 1.29 and 460 nM when it was in the 1st position. This nanobody was effective at blocking CXCR4-tropic HIV1 strain NL4.3 and the CCR5-tropic BaL, and its bi-specific construction was extremely potent in inhibiting HIV-1 X4 virus replication, especially when evaluated in PHA-stimulated PBMCs. This antibody was also used to construct bi-specific nanobodies with Ab04390 and Ab04391 (anti-IL23R [150D02] and [150H07]) and Ab04392 and Ab04393 (Anti-IL12RB1 [148C09] and [148F09]). In these bi-specific constructions, it served as an anchor (US20220242958).

Antibody first published in: PMID: Note on publication: The original publication describes the construction and characterization of various bi-specific antibodies.

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