United Kingdom (UK)
Recombinant monoclonal antibody to CD4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OX-38.
UniProt Accession Number of Target Protein: P05540
Alternative Name(s) of Target: W3/25 antigen; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3;
Immunogen: The original antibody was generated by immunizing mice with rat lymphocytes or rat thymocyte glycoproteins.
Specificity: This antibody binds rat CD4 cell surface antigen. CD4 is a transmembrane glycoprotein expressed on T helper cells, thymocytes, monocytes and macrophages. It plays an important role in the response of T helper cells to antigen. This antibody binds an identical or adjacent epitope to that bound by W3/25 antibody, and competes with mouse anti-rat CD4 antibody W3/25 for binding rat CD4.
Application Notes: This antibody can be used to identify CD4 antigen present on T cells, thymocytes, monocytes etc. using flow cytometry. This antibody was also able to immunoprecipitate detergent extracts of iodine 125 labeled thymocytes (PMID: 3159821). This antibody can be used for the in vitro depletion of T helper cells from slice cultures in studies looking for myelin loss in multiple sclerosis, the observed disruption being abrogated in these experimental conditions by T-cell depletion (PMID: 25500111). This antibody was tested for its ability to prolong the survival of heterotopic vascularized rat heart allografts transplanted across major histocompatibility barriers. Nine of 12 treated rats have maintained heterotopic abdominal heart allografts for greater than 175 days (PMID: 1976282). This antibody was used in a study to demonstrate that monoclonal antibody-induced depletion of CD4 helper/inducer T lymphocytes before transplantation of a fully mismatched heart allograft allows permanent engraftment in rats without further immunosuppression (PMID: 1977898).
Antibody first published in:
Jefferies et al. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med. 1985 Jul 1;162(1):117-27. PMID:3159821
Note on publication:
Describes the generation of this antibody and its use in the characterization of the CD4 (W3/25) antigen present on rat peritoneal macrophages.
Flow cytometry using anti-CD51 antibody NKI-M9 (Ab02884). HeLa cells were stained with the anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of NKI-M9 (Ab02884-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of rat splenocytes with anti-CD4 antibody OX-38 (Ab03050). Immunofluorescence analysis of paraformaldehyde fixed rat splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of OX-38 (Ab03050-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03050-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.