United Kingdom (UK)
Recombinant monoclonal antibody to CD44. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma F8:33-6-8-10.
UniProt Accession Number of Target Protein: P16070
Alternative Name(s) of Target: CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1
Immunogen: The original mouse antibody was raised by immunizing C57BL/6 mice with soluble CD44vRA. CD44vRA is a form of CD44, containing an extra alanine, derived from rheumatoid arthritis patients.
Specificity: This antibody is specific for CD44vRA, which is a CD44 derived from rheumatoid arthritis patients. CD44vRA contains an extra alanine. At higher concentration it will also bind to other isoforms of CD44. CD44 is cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment.
Application Notes: This antibody is recommended for the detection of CD44 from patients with rheumatoid arthritis (CD44vRA). Transwell migration assay was preformed to assess the specificity of the mouse version of this antibody. The antibody prevented namalwa-CD44vRA cells from crossing but did not prevent namalwa cells transfected with other forms of CD4 from crossing (US2011207678). To test the ability of this antibody to bind to CD44vRA, flowcytometry was preformed on Namalwa cells expressing different isoforms of CD44 (US2011207678).
Antibody first published in: CD44 polypeptides, polynucleotides encoding same, antibodies directed thereagainst and method of using same for diagnosing and treating inflammatory diseases US2011207678 PMID: Note on publication: Describes the generation and characterization of this antibody.