United Kingdom (UK)
Recombinant monoclonal antibody to CD47. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma C5D5.
UniProt Accession Number of Target Protein: Q08722
Alternative Name(s) of Target: C5/D5; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6
Immunogen: This antibody was raised by immunising Balb/c mice with T84 epithelial membranes.
Specificity: This antibody recongises human CD47 extracellular domain. CD47 is a a multitransmembrane glycoprotein widely expressed on all hematopoietic cells and most other cell types. It is involved in cell adhesion, Ca2+ influx, platelet activation and peripheral mononuclear neutrophils migration across endothelial and epithelial monolayers.
Application Notes: C5/D5 is recommended for staining cell surface CD47 as well as neutralising CD47. Furthermore, it is capable of inhibiting peripheral mononuclear neutrophils migration across polarised monolayers of the human intestinal epithelial cell line T84 in a bidirectional fashion (Parkos et al., 1996). This antibody is also very effective in detecting CD47 in flow cytometry; this was demonstrated with various cells, such as CaCO2 and polymorphonuclear neutrophils (Liu et al., 2001). Finally, C5D5 was also utilised in the immunofluorescence staining of the basolateral surfaces of methanol-fixed CaCO2 monolayers expressing exogenously transfected human CD47 (Liu et al., 2001).
Antibody first published in:
Parkos et al. CD47 mediates post-adhesive events required for neutrophil migration across polarized intestinal epithelia. J Cell Biol. 1996 Feb;132(3):437-50. PMID:8636220
Note on publication:
This article describes the generation and characterisation of C5D5 antibody.