Anti-CD63 [NK-1-C3]

Name: Anti-CD63 [NK-1-C3]
SKU: Ab01544
Availability: InStock

Description:

Recombinant monoclonal antibody to CD63. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma NK-1-C3.

Clone Name:

NK-1-C3

Published Application(s):

IP, WB, FC, IF, IHC

Published Species Reactivity:

Human, Mouse

Products based on this clone:

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Ab01544-1.1 Anti-CD63 [NK-1-C3]
Human, Mouse
Mouse IgG1
Purified
In Stock

Ab01544-23.0 Anti-CD63 [NK-1-C3]
Human, Mouse
Rabbit IgG
Purified
In Stock

Ab01544-10.0 Anti-CD63 [NK-1-C3]
Human, Mouse
Human IgG1
Purified
Ships in 4-5 weeks

Ab01544-10.3 Anti-CD63 [NK-1-C3]
Human, Mouse
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01544-13.12 Anti-CD63 [NK-1-C3]
Human, Mouse
Human IgG4-S228P
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P08962

Alternative Name(s) of Target: Granulophysin; LAMP-3; Tetraspanin-30; Tspan-30; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; CD63 antigen; NK1/C3; NKI/C3; NK-1.C3; NK1C3
Immunogen: This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.

Specificity: This antibody recognizes CD63. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation and plays a role in the activation of cellular signaling cascades.

Application Notes: The antibody NK-1-C3 has been widely used for the detection and analysis of CD63 via various methods. For instance, it was utilized as a marker of late endosomes and exosomes and the expression of CD63 was analysed via flow cytometry (Wang et al., 2019). Furthermore, NK-1-C3 (NK1/C3) proved successfull in labelling CD63 in multiple tissues, for example, when localizing CD63 in murine retinal pigment epithelium (RPE)/choroid tissue (Wang et al., 2009). Similarly, this antibody was used to detect CD63 on melanoma cells via immunofluorescence staining (Khazen et al., 2016).

Antibody first published in: Vennegoor et al. Biochemical characterization and cellular localization of a formalin-resistant melanoma-associated antigen reacting with monoclonal antibody NKI/C-3. Int J Cancer. 1985 Mar 15;35(3):287-95. PMID:3882581
Note on publication: This article describes the generation and characterization of this anntibody (NKI/C-3).

Western blot on K562 cell lysate using Ab01544-23.0. Click on image for more details.

Western Blot using anti-CD63 antibody NK-1-C3 (Ab01544) K562 cell lysate (35µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of NK-1-C3 (Ab01544-23.0) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

IF staining of A431 cells using Ab01544-23.0. Click on image for more details.

Immunofluorescence staining of fixed A431 cells with anti-CD63 antibody NK-1-C3 (Ab01544) Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of NK-1-C3 (Ab01544-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01544-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.