United Kingdom (UK)
Recombinant monoclonal antibody to CD7. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma T3-3A1.
UniProt Accession Number of Target Protein: P09564
Alternative Name(s) of Target: GP40; TP41; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9 ; T3.3A1; 3A1
Immunogen: The original antibody was generated by immunizing female BALB/c mice both intravenously and intraperitoneally with HSB-2 T cells.
Specificity: This antibody binds human CD7. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. CD7 is useful in the identification of precursor T cells as well as an important marker for the identification of leukemic T cells.
Application Notes: This antibody is capable of recognizing CD7 antigen present on various T cell lines like n HSB-2, HPB-MLT, MOLT-4, CEM, and TALL-1. This antibody also bound a majority (85%) of human PB E-rosette-positive cells. Immunoprecipitation of an HSB-2 extract with this antibody yielded one specific band with a molecular weight of approximately 40,000 in the presence of reducing agent (PMID: 6156457). This antibody was also used in the development of a cell ELISA to monitor the PHA-induced expression of the CD7 antigen in fixed mononuclear leukocytes (PMID: 3492562). This antibody was also used in combination with another anti-CD7 antibody mab 69 of development of a sandwich ELISA for detection of solubilized CD7 antigen (PMID: 2464646). This antibody was used in a study relating to identifying CD7 as target structure for T-cell imaging. Noninvasive CD7 immuno-imaging is a promising strategy to track T-cell responses in the context of diverse immunotherapies in vivo with additional theragnostic potential in T-cell-mediated diseases (PMID: 30613283).
Antibody first published in:
Haynes et al. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc Natl Acad Sci U S A. 1979 Nov; 76(11): 5829–5833. PMID:316538
Note on publication:
Describes the generation of this antibody and uses it for the study of CD7 positive and negative T cell subsets in the regulation of normal and abnormal human immune responses.