United Kingdom (UK)
Recombinant monoclonal antibody to CD79b. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma B29/123.
UniProt Accession Number of Target Protein: P40259
Alternative Name(s) of Target: B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; Ig-beta; Immunoglobulin-associated B29 protein; B29; IGBAGM6; B29 123
Immunogen: This antibody was raised against the sequence G-E-V-K-W-S-V-G-E-H-P-G-Q-E, representing the carboxy-terminus, from amino acid 215 on, of the human and murine B29 polypeptides.
Specificity: This antibody recognises intracellular C-terminus of human and murine CD79b which is part of the B-cell antigen receptor which constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells.
Application Notes: This antibody is recommended for the immunohistochemical and Western blot investigation of B-cells and their cancers which express CD79b. B29/123 antibody recognises specifically CD79b protein whose expression pattern during B-cell development is distinct from CD79a. Consequently, this antibody might be utilised in the investigation of processes associated with characteristic CD79b expression. For instance, one group used it in the immunofluorescence staining of WEHI231 cells transduced with LAPTM5-IRES-GFP in a study on B-cell activation (Ouchida et al., 2010).
Antibody first published in:
Brouns et al. The structure of the mu/pseudo light chain complex on human pre-B cells is consistent with a function in signal transduction. Eur J Immunol. 1993 May;23(5):1088-97. PMID:8477803
Note on publication:
This article describes the generation and characterisation of B29/123 antibody.
Western Blot using anti-CD79b antibody B29/123 (Ab01333) Daudi cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of B29/123 (Ab01333-23.0) at 0.0003 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunofluorescence staining of fixed Daudi cells with anti-CD79b antibody B29/123 (Ab01333) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of B29/123 (Ab01333-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01333-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-CD79b antibody B29/123 (Ab01333). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of B29/123 (Ab01333-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.