United Kingdom (UK)
Recombinant monoclonal antibody to CD80. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the heterohybridoma 7C10.
UniProt Accession Number of Target Protein: P33681
Alternative Name(s) of Target: B7.1; CD28L; CD28LG; B7-1; B7; BB1; B7/BB1;T-lymphocyte activation antigen CD80; Activation B7-1 antigen; CTLA-4 counter-receptor B7.1; CTLA4L; CTLA-4L
Immunogen: This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen.
Specificity: This antibody binds human CD80 and inhibits the B7.1:CD28 pathway.
Application Notes: This antibody can be used for identification of human B7.1 antigen and is capable of inhibiting the binding of B7.1 to CD28, however it does not block the binding of B7.1 to CTLA-4. This antibody further inhibits the production of IL-2. Blocking of the primary activation signal between CD28 and B7.1 (CD80) with these antibodies while allowing the unimpaired or coincident interaction of CTLA-4 and B7.1 represents a combined antagonistic effect on positive co-stimulation with an agonistic effect on negative signaling. The binding of the antibody to soluble B7.1 was tested using ELISA. This antibody tested positive for binding to human C1q protein. This antibody is reported to cause keratinocyte hyperproliferation and decrease in psoriasis by inhibiting the B7:CD28 pathway. This antibody is further reported to be useful for the treatment of intestinal inflammation and rheumatoid arthritis.
Antibody first published in: PMID:
Flow cytometry using the Anti-CD80 antibody 7C10 (Ab01687). Paraformaldehyde fixed Daudi cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 7C10 (Ab01687-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of Daudi cells with anti-CD80 (Ab01687) 7C10 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of 7C10 (Ab01687-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01687-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.