United Kingdom (UK)
Recombinant monoclonal antibody to CD81. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Ab10.
UniProt Accession Number of Target Protein: P60033
Alternative Name(s) of Target: CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28 (Tspan-28)
Immunogen: The original antibody was generated by immunization of Balb/c and Naval Medical Research Institute (NMRI) mice with CD81 extracellular loop 2 (ECL2) protein.
Specificity: This antibody is specific for the large extracellular loop of human CD81.
Application Notes: The scFv version of this antibody is based on an anti-CD81 clone whose binding specificity was determined via FACS that was successfully used in an inhibition assay against HCV (Ji et al., 2015; PMID: 25417967). The exact binding site of this antibody was determined via crystallization (Nelson et al., 2018; PMID: 29778602). The scFv version of this antibody was used successfully in FC (Susa et al., 2020; PMID: 32338599).
Antibody first published in:
Nelson et al. Structure-Guided Combinatorial Engineering Facilitates Affinity and Specificity Optimization of Anti-CD81 Antibodies J Mol Biol. 2018 Jul 6;430(14):2139-2152. doi: 10.1016/j.jmb.2018.05.018. Epub 2018 May 17 PMID:29778602
Note on publication:
The original publication explores the development of antibodies targeting the host antigen CD81 to block hepatitis C viral entry.