United Kingdom (UK)
Recombinant monoclonal antibody to CD83. Manufactured using AbAbâs Recombinant Platform with variable regions (i.e. specificity) from the hybrodoma 159.
UniProt Accession Number of Target Protein: A0A1L1RYL9
Alternative Name(s) of Target: chicken CD83; chCD83; chCD83-159; Ig-like domain-containing protein
Immunogen: The original antibody was raised by immunizing BALB/c mice with purified chicken DC83 protein.
Specificity: The antibody binds specifically to chicken CD83, a membrane-bound glycoprotein belonging to the immunoglobulin superfamily that is primarily expressed on mature dendritic cells (DCs). The antibody did not react with chicken CD80.
Application Notes: This antibody was used for detection of chicken CD83 expressed in CHO cells using flow cytometry. The antibody also reacted with HD11 cells by flow cytometry. The antibody recognized CD83 protein on Western blots of lysates from lipopolysaccharide-stimulated spleen mononuclear cells or unstimulated HD11 cells (Lee et al, 2012; pmid: 22197010).
Antibody first published in:
Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken CD83. Vet Immunol Immunopathol. 2012;145(1â2):527â533. PMID:22197010
Note on publication:
The paper describes the generation and characterization of an antibody against chCD83 for the purpose of defining its immunological, cellular, and biochemical characteristics.