United Kingdom (UK)
Recombinant monoclonal antibody to CEA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BW431/26.
UniProt Accession Number of Target Protein: P06731
Alternative Name(s) of Target: Carcinoembryonic antigen; Carcinoembryonic antigen-related cell adhesion molecule 5; CEACAM5; Meconium antigen 100; CD66e
Immunogen: The original antibody was generated by immunizing mice with CEA positive adenocarcinoma cells preferably from lungs.
Specificity: This antibody recognizes and binds human carcinoembryonic antigen in its purified state as well as on tissue sections.
Application Notes: Technetium 99-labeled (Tc-99m) BW431/26 has been used in the diagnosis and detection of colorectal carcinomas, breast cancers, bladder carcinomas, lung adenocarcinomas and their recurrences by immunoscintigraphy (PMID: 2602902, 2662074, 2685692, 2297747, 2157833, 2166552, 2383485, 1984945 ). The use of Tc-99m-BW 431/26 immunoconjugates in immunoscintigraphy resulted in reduction of radiation dose for the patient down to 5% of the dose applied as compared with I-131 labelled MAbs (PMID: 3262953). Humanized version of this antibody also shows similar binding affinity as compared to its murine parent antibody (PMID: 1762576).
Antibody first published in: US6294172 PMID:
Immunofluorescence staining of MCF7 cells with anti-CEA (Ab02064) BW431/26 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of BW431/26 (Ab02064-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cell junction staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02064-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.