United Kingdom (UK)
Recombinant monoclonal antibody to Cell surface-binding protein D8L. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma LA5.
UniProt Accession Number of Target Protein: P04195
Alternative Name(s) of Target: OPG105; D8L; VACWR113; Carbonic anhydrase homolog; Cell surface-binding protein OPG105
Immunogen: The original antibody was raised by immunizing a mouse with VACV.
Specificity: This antibody is specific for protein D8 of the vaccinia virus. It binds above the CS-E binding crevice
Application Notes: The original version of this antibody was used for ELISA on recombinant D8 protein. The binding strength of this antibody was also determined using surface plasmon resonance on VAVC D8 (Matho et al., 2012; PMID:22623786). The original antibody was used for neutralization of a intracellular mature virion. It showed that is was only able to neutralize the intracellular mature virion in the present of complement (Matho et al., 2018; PMID:29123031).
Antibody first published in:
Matho et al. Structural and biochemical characterization of the vaccinia virus envelope protein D8 and its recognition by the antibody LA5 J Virol. 2012 Aug;86(15):8050-8. PMID:22623786
Note on publication:
The original paper describes the biochemical and structural characterization of one of the immunodominant vaccinia virus (VACV) antigen, D8, and its binding to the monoclonal antibody LA5.