United Kingdom (UK)
Recombinant monoclonal antibody to Citrus tristeza virus coat protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3DF1.
UniProt Accession Number of Target Protein: Q00686
Alternative Name(s) of Target: CP; CTV; CTV capsid protein; CTV coat protein; capsid protein, coat protein; p25 major coat protein
Immunogen: The original antibody was generated by immunizing BALB/c mice intraperitoneally with Citrus tristeza virus (isolate T-308) preparation.
Specificity: This antibody binds the p25 major coat protein of the Citrus tristeza virus (CTV). This antibody cannot bind the CTV isolate T-397-P. Tristeza is the most important virus disease of citrus worldwide. It is caused by citrus tristeza virus (CTV), a closterovirus that is naturally spread in the fields by aphids in a semipersistent manner and is also easily transmitted by grafting. CTV is a monopartite, single stranded RNA virus encapsulated in a coat protein.
Application Notes: This antibody can be used for the detection of Citrus tristeza virus in crude extracts of plants infected with isolates T-300 or T-308 using ELISA and double antibody sandwich ELISA. This antibody reacted with 20 CTV isolates from different Spanish citrus areas as well as with CTV isolates from California (T-514) and two unclassified from Iran and Vietnam in an ELISA (Vela et al., 1986). Transgenic plants expressing this antibody showed both a delay in symptom appearance and attenuation of symptom intensity. Overall, 40–60% of the plants were resistant, as revealed by the absence of virus antigens in tissue-print ELISA (PMID: 20204695).
Antibody first published in:
Vela et al. Production and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis. J. gen. Virol. (1986), 67, 91-96. PMID:
Note on publication:
Describes the generation and characterization of two mouse antibodies against Citrus tristeza virus.