United Kingdom (UK)
Recombinant monoclonal antibody to Complement C3. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from phage display MF9.
UniProt Accession Number of Target Protein: P12387
Alternative Name(s) of Target: C3
Immunogen: A female New Zealand White rabbit was immunised with guinea pig C3 by three subsequent subcutaneous injections. The RNA was isolated from the spleen, a phage display library constructed and then used to produce soluble scFv fragments.
Specificity: MF9 is highly specific for guinea pig C3, with no binding observed between MF9 and guinea pig C3a, rat C3 and human C3.
Application Notes: ELISA was used to confirm the specificity of MF9 for guinea pig C3, with MF9 displaying high reactivity for gp C3. Reactivity with guinea pig C3a, rat C3, and human C3 was not observed (Hawlisch et al, 2000). MF9 was used to stain a rejected transplanted liver in guinea pigs (Hawlisch et al, 2000).
Antibody first published in:
Hawlisch et al, 2000. Guinea pig C3 specific rabbit single chain Fv antibodies from bone marrow, spleen and blood derived phage libraries J Immunol Methods. 2000 Mar 6;236(1-2):117-31 PMID:10699585
Note on publication:
Describes the original generation of MF9 and its characterisation through ELISA and IHC.