United Kingdom (UK)
Recombinant monoclonal antibody to complete inactivated virus. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Nb93.
Alternative Name(s) of Target: H5N1 virus
Immunogen: The original antibody was generated by immunizing Bactrian camel (Camelus bactrianus) with inactivated influenza H5N1 virus, followed by antibody library construction and phage display-based selection.
Specificity: This antibody specifically binds the H5N1 virus. It does not bind to the other subtypes of influenza A viruses.
Application Notes: The specificity of this antibody (VHH) was confirmed in ELISA analysis. The antibody was used as a detecting antibody in sandwich ELISA, where it was used in a matched pair in conjunction with Nb7; the detection limit of this method for H5N1 virus was 14.1 ng/mL. Sandwich ELISA was employed for H5N1 detection in serum samples (Zhu et al, 2014; pmid:25526777).
Antibody first published in:
Zhu et al. Streptavidin-biotin-based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1 J Transl Med. 2014 Dec 20;12:352. PMID:25526777
Note on publication:
The paper describes the generation and characterization of the antibody.