United Kingdom (UK)
Recombinant monoclonal antibody to Cyclosporin A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma R454511.
Alternative Name(s) of Target: CsA; ciclosporin A, cyclosporine A
Immunogen: BALB/c mice were immunised with a D-Lys8-Cs derivative coupled to a carrier protein.
Specificity: R454511 binds to residues 1, 2, 3, 4, 5, 9 and 11 of CsA.
Application Notes: Mutagenesis of Cs-derivatives identified that R454511 binds to residues 1, 2, 3, 4, 5, 9 and 11 of CsA (Quesniaux et al., 1990). In ELISA experimenets, R454511 was found to bind to Cs conjugated with both Thr2 (Thr2-Cs-BSA) and Lys8 (D-Lys8-Cs-BSA) (Quesniaux et al., 1990). The crystal structure of R454511 in complex with CsA has been obtained to 2.65 Å resolution (Altschuh et al., 1992).
Antibody first published in:
Quesniaux et al, 1990. Monoclonal antibodies to cyclosporin are representative of the major antibody populations present in antisera of immunized mice. Mol Immunol. 1990 Mar;27(3):227-36. PMID:2342487
Note on publication:
Describes the original generation of the antibody and its characterisation through ELISA.