United Kingdom (UK)
Recombinant monoclonal antibody to Cytokeratin 8. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma TS1.
UniProt Accession Number of Target Protein: P05787
Alternative Name(s) of Target: CK8; CK-8; CK 8; KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; Type-II keratin Kb8; TS 1; Mab 202
Immunogen: The original antibody was generated by immunizing BALB/c mice with purified human gastrointestinal tumor derived cytokeratin.
Specificity: This antibody binds a discontinuous epitope on helical 2B domain of the human cytokeratin 8 between amino acid residues 340–365 (QRGELAIKDANAKLSELEAALQRAKQ). Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Together with KRT19, cytokeratin 8 helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin 8 is a serological tumor marker
Application Notes: This antibody has specific reactivity for human cytokeratin 8. The binding characterization and epitope mapping of this antibody was done using ELISA (PMID: 9486565; 9892182). This antibody was also capable of immunoprecipitating human cytokeratin 8 from a sample. This antibody can be used in the identification of cytokeratin 8 in a western blot assay. This antibody was also used in the immunohistochemical staining of frozen sections of normal human adult tissues like skin, esophagus and liver (PMID: 2479674). This antibody is capable of binding cytokeratin 8 expressed on carcinomas in vivo. This antibody was used for the radio immunolocalization of cytokeratin 8 and radioimmunotherapy (PMID: 9892182; 7493362; 9406704).
Antibody first published in:
Sundström et al. Diversity in immunoreactivity of tumor-derived cytokeratin monoclonal antibodies. J Histochem Cytochem. 1989 Dec;37(12):1845-54. PMID:2479674
Note on publication:
Describes the generation and characterization of 10 antibodies against cytokeratin which are expressed on normal and malignant epithelial cells.
Flow cytometry using anti-Cytokeratin 8 antibody TS1 (Ab03179). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of TS1 (Ab03179-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.
Western blot using anti-Cytokeratin 8 antibody TS1 (Ab03179). A431 cells lysate (35µg protein in RIPA buffer) was resolved on an SDS-PAGE gel and blots were probed with the chimeric rabbit version of TS1 (Ab03179-23.0) at 0.3µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunofluorescence staining of HeLa cells with anti-Cytokeratin 8 antibody TS1 (Ab03179). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of TS1 (Ab03179-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03179-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.