Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]

Description:

Recombinant monoclonal antibody to Cytotoxin 1, 3, 7. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody TPL0027_01_F7.

Clone Name:

TPL0027_01_F7

Published Application(s):

ELISA

Published Species Reactivity:

Naja nigricollis

8

Products based on this clone:

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Ab01642-30.11 Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]
Naja nigricollis
scFv fragment (His)
ScFv
Purified
Ships in 4-5 weeks

Ab01642-32.1 Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]
Naja nigricollis
Sheep IgG
Purified
Ships in 4-5 weeks

Ab01642-1.1 Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]
Naja nigricollis
Mouse IgG1
Purified
In Stock

Ab01642-21.0 Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]
Naja nigricollis
Mouse IgM
Purified
Ships in 4-5 weeks

Ab01642-23.0 Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]
Naja nigricollis
Rabbit IgG
Purified
In Stock

Ab01642-10.0 Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]
Naja nigricollis
Human IgG1
Purified
In Stock

Ab01642-10.3 Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]
Naja nigricollis
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01642-15.0 Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]
Naja nigricollis
Human IgM
Purified
Ships in 4-5 weeks

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Alternative Name(s) of Target: CTX1,3,7; CX1; Cardiotoxin F8; Cardiotoxin-I; CTX-I; Toxin CM-6; Cytotoxin 3; CX3; Toxin CM-7; Cytotoxin 7; Toxin CM-4B
Immunogen: The antibody was raised using IONTAS phage display library, a naïve human scFv library consisting of 4.0 · 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind a toxin, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind.

Specificity: TPL0027_01_F7 antibody recognizes cytotoxins 1, 3 and 7, which are some of the major toxins in the venom of the black-necked spitting cobra (Naja nigricollis).

Application Notes: TPL0027_01_F7 antibody has high affinity to the cytotoxins 1, 3 and 7. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibody's high affinity was confirmed by its ability to bind the target proteins in a venom fraction even at 1:32,000 dilution (3.125 · 10^-2) µg/mL (Jürgensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites.

Antibody first published in: Jürgensen et al. Harnessing monoclonal antibodies for development of a specific treatment against Naja nigricollis envenoming. 10.13140/RG.2.2.24771.14884. PMID:
Note on publication: This poster describes the generation and characterization of the TPL0027_01_F7 antibody among other antibodies directed against the venom toxins of Naja nigricollis.