United Kingdom (UK)
Recombinant monoclonal antibody to Desmoglein 3 . Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AK23.
UniProt Accession Number of Target Protein: P32926
Alternative Name(s) of Target: DSG3; PVA; CDHF6; Desmoglein-3; 130 kDa pemphigus vulgaris antigen; Cadherin family member 6
Immunogen: The original antibody was generated by immunizing Dsg3 knockout mice with recombinant mouse Dsg3-His in complete Freund's adjuvant subcutaneously.
Specificity: This antibody binds mouse Dsg3 and also shows reactivity for human Dsg3. It does not show any affinity towards Dsg1. AK23 recognizes a calcium-dependent conformational epitope on Dsg3, which consisted of the V3, K7, P8, and D59 Dsg3-specific residues that formed the adhesive interface between juxtaposed Dsg.
Application Notes: This antibody can be used to study the blister formation and complex interaction that occur in autoimmune blistering disease like Pemphigus vulgaris (PV). Pemphigus vulgaris (PV) is an epidermal blistering disorder caused by antibodies directed against the desmosomal cadherin desmoglein-3 (Dsg3). AK23 was reported to induce pemphigus vulgaris (PV) blisters in neonatal mice. The binding affinity of this antibody for human and mouse desmoglein 3 was determined using ELISA. This antibody was also used in the live keratinocyte staining of mouse PAM212 cell line and indirect immunofluorescent staining of mouse oral mucuous membrance, mouse liver and lower parts of the human epidermis. Immunoprecipitaton was used for the determination of the epitope of the antibody (PMID: 12574390). This antibody was also used in the study of alteration in desmosome morphology (PMID: 23226536).
Antibody first published in:
Tsunoda et al. Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3. J Immunol. 2003 Feb 15;170(4):2170-8. PMID:12574390
Note on publication:
Describes the generation of this antibody in pemphigus vulgaris mouse model and examines its pathogenic activity in the induction of blister formation.
Immunofluorescence staining of A431 cells with Anti-Desmoglein 3 (Ab02730) AK23 Immunofluorescence analysis of paraformaldehyde fixed A431 cells stained with the chimeric mouse IgG1 version of AK23 (Ab02730-1.1) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02730-1.1, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-Desmoglein 3 antibody AK23 (Ab02730). Paraformaldehyde fixed A431 cells were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of AK23 (Ab02730-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.