United Kingdom (UK)
Recombinant monoclonal antibody to DLL3. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SC16.25.
UniProt Accession Number of Target Protein: Q9NYJ7
Alternative Name(s) of Target: Delta-like protein 3; Drosophila Delta homolog 3 (Delta3)
Immunogen: The original antibody was generated by immunizing three strains of mice (Balb/c, CD-1 and FVB) with recombinant human DLL3-Fc or with human DLL3-His fusion constructs.
Specificity: This antibody binds human DLL3 in the N-terminal domain. Cross-reacts with cynomolgus monkey DLL3 with no binding to mouse/rat DLL3. Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL3 consists of a 466 amino acid (aa) extracellular domain (ECD) with one DSL domain and six EGF-like repeats, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain. It is an inhibitory Notch ligand that is highly expressed in small cell lung cancer (SCLC) and other neuroendocrine tumors but minimally expressed in normal tissues. It is therefore being explored as a potential therapeutic target in SCLC.
Application Notes: The binding characterization of this antibody to human DLL3 was done using ELISA. Binding affinity of mouse IgG antibody for human DLL3 is Kd= less than 0.2 nM. This antibody can bind human and cynomolgus monkey DLL3 expressed on surface of 293 cells via flow cytometry. This antibody can mediate killing of tumor cells expressing DLL3. Humanized version of this antibody is also available on request (US9089615).
Antibody first published in: PMID: