United Kingdom (UK)
Recombinant monoclonal antibody to Dll4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HMD4-2.
UniProt Accession Number of Target Protein: Q9JI71
Alternative Name(s) of Target: Delta4; Delta-like protein 4; Delta 4; Drosophila Delta homolog 4
Immunogen: The original hamster IgG version of this antibody was raised by immunizing Armenian hamsters (Oriental Yeast) with a recombinant mouse Dll4 via intraperitoneal injections and subsequent generation of the hybridoma cells.
Specificity: HMD4-2 recognizes murine Dll4, one of the five major ligands of the Notch signalling pathway. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumours, and pancreatic, bladder and colonic cancer.
Application Notes: This antibody can be used to investigate the role of the signalling pathways related to the Notch receptors and its ligands. The antibody blocks Notch1-Fc and Notch4-Fc binding to mouse Dll4-expressing cells in a dose-dependent manner and does not bind to cells expressing other mouse Notch ligands, such as Dll1, Jagged1, or Jagged2 (Sekine et al., 2009; 19181931). HMD4-2 was demonstrated as an effective infibitor able to neutralize the Dll4-dependent Notch signalling (Sekine et al., 2009; 19181931). It was used to block Dll4-mediated Notch signaling in LDL-receptor–deficient (Ldlr−/−) mice fed a high-fat, high-cholesterol diet, a model that produces atherosclerosis and metabolic disturbances resembling those seen in the cardiometabolic syndrome in order to test the hypothesis in vivo that Dll4 contributes to the pathogenesis of the cardiometabolic syndrome (Fukuda et al., 2012; pmid: 22699504). This study shown that the Dll4 blockage inhibits atherosclerosis and metabolic disorders (Fukuda et al., 2012; pmid: 22699504). This clone was also utilized in flow cytometry to analyse the expression of Dll4 on splenocytes, dendritic cells and macrophages (Moriyama et al., 2008; pmid: 18381350; Sekine et al., 2009; 19181931). Further, it was also used in the immunohistochemistry to stain frozen murine spleen samples (Sekine et al., 2009; 19181931). It was also used as a part of a control mixture in the functional studies on the maintenance of CD8- dendritic cells (Sekine et al., 2009; 19181931).
Antibody first published in:
Moriyama et al. Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice Int Immunol. 2008 Jun;20(6):763-73. doi: 10.1093/intimm/dxn034. Epub 2008 Apr 1. PMID:18381350
Note on publication:
Describes the generation and characterization of the HMD4-2 antibody.
Immunofluorescence staining of mouse splenocytes with anti-Dll4 (Ab02089) HMD4-2 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of HMD4-2 (Ab02089-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02089-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.