United Kingdom (UK)
Recombinant monoclonal antibody to Ebola GP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 13F6-1-2.
UniProt Accession Number of Target Protein: Q05320
Alternative Name(s) of Target: GP; Envelope glycoprotein
Immunogen: This antibody was raised by immunizing mice with VEE virus replicon particles (VRP) that express Ebola GP (EboGP-VRP).
Specificity: This antibody is specific for amino acids 401-417 of the ebola glycoprotein. Ebola is a viral hemorrhagic fever of humans and other primates caused by ebolaviruses.
Application Notes: This antibody recognizes heavily glycosylated mucin-like domain of the Ebola virus virion-attached glycoprotein (GP) and protects animals against lethal viral challenge. In order to determine the protective efficacy of this antibody, the mouse version of this antibody was evaluated for their ability to protect mice from a lethal Ebola challenge. The characteristics were further analyzed using radioimmunoprecipitation, western blot and peptide-binding analysis. These assays were performed using the mouse version of this antibody and Ebola GP as antigen. Furthermore, the ability of the mouse version of this antibody to inhibit the Ebola virus from forming plaques was also determined. This is an indication that this antibody can neutralize the Ebola virus (US6875433). This antibody was expressed in plants and to show its functionality western blot was performed with the mouse version of this antibody (which was expressed in a plant) on Ebola virus like particles. Further the mouse version of this antibody was also used for ELISA on Ebola VLP. And finally to determine the binding affinity of the mouse version of this antibody surface plasmon resonance was performed using Ebola VLP as antigen (Lim et al, 2020; pmid:32977599). To assess the specific binding of this antibody an ELISA on Ebola GP was performed using the scFv version of this antibody (Rodriguez-Martinez et al, 2015; pmid:26489048). The structure of the mouse version of this antibody was determined using molecular replacement. Further the binding affinity of the mouse version of this antibody was determined using surface plasmon resonance on Ebola virus bound to BSA (Lee et al, 2008; pmid:18005986).
Antibody first published in:
Lee et al. Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain. J Mol Biol. 2008 Jan 4;375(1):202-16. doi: 10.1016/j.jmb.2007.10.017. Epub 2007 Oct 16. PMID:18005986
Note on publication:
The crystal structure, at 2.0 A, of 13F6-1-2 in complex with its Ebola virus GP peptide epitope is presented.