- Ab00133-2.0 Anti-EGFR 
- Mouse IgG2a
- In Stock
- Ab00133-23.0 Anti-EGFR 
- Rabbit IgG
- In Stock
Recombinant monoclonal antibody to EGFR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 528.
UniProt Accession Number of Target Protein: P00533
Alternative Name(s) of Target: ERBB; ERBB1; HER1; PIG61; mENA; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; epidermal growth f
Immunogen: Partially purified EGFR from A431 cells.
Specificity: Recognizes the extracellular domain of EGFR.
Antibody first published in:
Kawamoto T, Sato JD, Le A, Polikoff J, Sato GH, Mendelsohn J. Growth stimulation of A431 cells by epidermal growth factor: identification of high-affinity receptors for epidermal growth factor by an anti-receptor monoclonal antibody. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1337-41. PMID:6298788
Note on publication: Describes the generation of the antibody and its inhibitory effect on A431 cell growth.
Flow-cytometry using the anti-EGFR antibody 528 (Ab00133) HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 528 (Ab00133-23.0, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analysed on a FACSCanto flow-cytometer.
Immunofluoresence staining of fixed HeLa cells with anti-EGFR antibody 528 (Ab00133) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of Ab00133 at 10µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00133-23.0, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.