United Kingdom (UK)
Recombinant monoclonal antibody to envelope protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody ZIKVEF2L01.
Alternative Name(s) of Target: ZIKV envelope protein; E protein
Immunogen: The original antibody was generated by immunizing chicken with ZIKV envelope protein followed by antibody library construction and phage display-based selection.
Specificity: The antibody binds to the E protein of Zika virus. The envelope protein forms a dimer on the smooth surface of the mature virus, and participates in receptor binding and fusion to the host.
Application Notes: The specificity of the scFv format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant E protein by western blot analysis. The scFv format recognized the E protein in a transfected Vero cell by flow cytometry. Competitive inhibition assay was employed to calculate the percentage of inhibition and the dissociation constant of the antibody against the E protein (inhibition of 50% = 0.55 ug/mL and Kd= 2.04 *10^-8 M). Therefore, the antibody has diagnostic or therapeutic potential for ZIKV (PMID: 31940993).
Antibody first published in:
Mwale et al. Expression, Purification, and Characterization of Anti-Zika virus Envelope Protein: Polyclonal and Chicken-Derived Single Chain Variable Fragment Antibodies Int J Mol Sci. 2020 Jan; 21(2): 492. PMID:31940993
Note on publication:
The paper describes the generation and characterization of a panel of antibodies against the E protein of Zika virus.