Anti-EpCAM [KS1/4]

Description:

Recombinant monoclonal antibody to EpCAM. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma KS1/4.

Clone Name:

KS1/4

Published Application(s):

Immunoperoxidase staining, ELISA, FC

Published Species Reactivity:

Human

6

Products based on this clone:

{{heading}}

Please Select

Ab01686-2.0 Anti-EpCAM [KS1/4]
Human
Mouse IgG2a
Purified
In Stock

Ab01686-3.0 Anti-EpCAM [KS1/4]
Human
Mouse IgG2b
Purified
Ships in 4-5 weeks

Ab01686-3.3 Anti-EpCAM [KS1/4]
Human
Mouse IgG2b
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01686-10.0 Anti-EpCAM [KS1/4]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab01686-10.3 Anti-EpCAM [KS1/4]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01686-23.0 Anti-EpCAM [KS1/4]
Human
Rabbit IgG
Purified
In Stock

Loading...

UniProt Accession Number of Target Protein: P16422

Alternative Name(s) of Target: CD326; Ep-CAM; KS1/4 antigen; KSA; Epithelial cell adhesion molecule; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; 17-1A-antigen; EGP314; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1.
Immunogen: The original antibody was generated by immunizing BALB/c mice with human adenocarcinoma of the lung cell line UCLA P3.

Specificity: This antibody binds to the EpCAM receptor which is expressed on surface of adenocarcinomas and almost all epithelial cell membranes except mesodermal and neural cell membranes.

Application Notes: This antibody has been shown to be an effective agent for the invitro detection of disease and invivo diagnosis, prognosis and treatment of adenocarcinoma. Initial specificity of the antibody was determined by ELISA. Immunoperoxidase staining and immunofluorescence staining techniques were used to identify the reactivity of the antibody with various tissues (PMID: 6362852). The monoclonal antibody-vinca alkaloid conjugate, KS1/4-DAVLB (LY256787) were administered to nude mice bearing P3/UCLA human lung adenocarcinoma tumors. It was shown that KS1/4 monoclonal antibody targets DAVLB to the P3/UCLA human lung adenocarcinoma in vivo in the human xenograft model and that an increased therapeutic index may be achieved with LY256787 over conventional free drug therapy. (PMID: 3494841). KS1/4-DAVLB conjugates were also used for site directed therapy of epithelial malignancies (PMID: 2979063). Flow cytometric methods were used to evaluate of the cell surface binding properties of monoclonal antibody (KS1/4)-drug/toxin conjugates (PMID: 3492560). Further characterization of the antibody via immunoperoxidase techniques, flow cytometric analyses and solid phase enzyme-linked immunoassays suggested that this antibody represents an epithelial malignancy marker (PMID: 3049306). Phase I clinical trials revealed that 10 out of eleven patients had human anti-mouse response (Elias et al., 1990). Humanized version of this antibody is available on request.

Antibody first published in: Varki et al. Antigens Associated with a Human Lung Adenocarcinoma Defined by Monoclonal Antibodies. Cancer Research (1984); Vol- 44; 681-687. PMID:6362852
Note on publication: Describes the generation and characterization of antibody.

Binding Curve

Western blot on A431, Caco-2, MCF7 and HepG2 cell lysate using Ab01686-23.0. Click on image for more details.

Binding Curve

Western Blot using Anti-EpCAM antibody KS1/4 (Ab01686). A431(A), Caco-2(B), MCF7(C) and HepG2(D) cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of KS1/4 (Ab01686-23.0) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Binding Curve

Flow cytometry analysis of Caco-2 cells using Ab01686-23.0. Click on image for more details.

Binding Curve

Flow cytometry using the Anti-EpCAM antibody KS1/4 (Ab01686). Paraformaldehyde fixed Caco-2 cells were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of KS1/4 (Ab01686-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Binding Curve

Western blot on A431, Caco-2, MCF7 and HepG2 cell lysate using Ab01686-23.0. Click on image for more details.

Binding Curve

Western Blot using Anti-EpCAM antibody KS1/4 (Ab01686). A431(A), Caco-2(B), MCF7(C) and HepG2(D) cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of KS1/4 (Ab01686-23.0) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Binding Curve

IF staining of Caco-2 cells using Ab01686-23.0. Click on image for more details.

Binding Curve

Immunofluorescence staining of Caco-2 cells with anti_EpCAM (Ab01686) KS1/4 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric rabbit IgG version of KS1/4 (Ab01686-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01686-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.