Anti-Equine IFN gamma [M14]

Description:

Recombinant monoclonal antibody to Equine IFN gamma. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma M14.

Clone Name:

M14

Published Application(s):

IP, WB, ELISA, FC, IF

Published Species Reactivity:

Horse

UniProt Accession Number of Target Protein: P42160

Alternative Name(s) of Target: IFN-g; IFN-gamma; interferon gamma; IFNG
Immunogen: This antibody was raised by immunising mice with recombinant equine IFN-gamma, expressed as a GST fusion protein, produced in E. coli.

Specificity: This antibody is specific for equine IFN gamma.

Application Notes: The specificity of this antibody for equine IFN-gamma has been confirmed in in ELISA and Western blot analysis using recombinant equine IFN-gamma derived from E. coli (Gutmann et al, 2005). This antibody has been used in Western blot and immunofluorescence analysis to detect equine IFN-gamma in mammalian cells transfected with IFN-gamma and insect cells infected with equine IFN-gamma-recombinant baculovirus (Gutmann et al, 2005).

Antibody first published in: Gutmann et al. Characterisation and quantification of equine interferon gamma Vet Immunol Immunopathol. 2005 Mar 10;104(1-2):105-15. PMID:15661336
Note on publication: Describes the original generation of this antibody, and its characterisation.

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