United Kingdom (UK)
Recombinant monoclonal antibody to ESAT-6. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the ribosome display antibody EA-S3.
UniProt Accession Number of Target Protein: B5TV89
Immunogen: BALB/c mice were immunised with ESAT-6 protein and RNA extracted from the spleen to produce a scFv library. Eukaryotic ribosome display was used to select EA-S3.
Specificity: ESAT-6 is a potent T-cell antigen and suggested as a diagnostic biomarker for TB infection.
Application Notes: ELISA was used to confirm the specificity of EA-S3 for ESAT-6; ELISA also determined the affinity constant of EA-S3 for ESAT-6 as 3.74x10^8 M^-1 (Ahangarzadeh et al, 2017).
Antibody first published in:
Ahangarzadeh et al, 2018. Selection of single-chain variable fragments specific for Mycobacterium tuberculosis ESAT-6 antigen using ribosome display. Iran J Basic Med Sci. 2017 Mar;20(3):327-333 PMID:28392906
Note on publication:
Describes the generation of EA-S3 from a ribosome display library and its characterisation through ELISA.