United Kingdom (UK)
Recombinant monoclonal antibody to Fab . Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody TP896.
Alternative Name(s) of Target: antigen-binding fragment; fragment antigen binding
Immunogen: This antibody was raised by immunising alpacas with 1.0 mg polyclonal rabbit IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Specificity: This antibody recognises Fab of the rabbit IgG.
Application Notes: TP896 nanobody can be used for the purification of antigens or antigen complexes previously bound to a primary antibody, as it successfully isolated polyclonal rabbit IgG from serum and then released it under physiological conditions using SUMOStar protease cleavage (Pleiner et al., 2018).
Antibody first published in:
Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID:29263082
Note on publication:
The article describes the generation and characterisation of this nanobody.