United Kingdom (UK)
Recombinant monoclonal antibody to Factor H binding protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the ribosome display clone4.
UniProt Accession Number of Target Protein: E6MV22
Alternative Name(s) of Target: fHbp; GNA1870; Genome-derived Neisseria antigen 1870; Lipoprotein 2086
Immunogen: The recombinant fHbp protein of Neisseria meningitidis was used as the antigen for the immunization of BALB/c mice. Anti-fHbp VH/k chain ribosome display libraries was generated and the actual antibody was obtained by panning the scFv library against the recombinant fHbp protein by using a single round of the ribosome display method via a rabbit reticulocyte lysate system.
Specificity: This antibody binds a surface exposed epitope on N. meningitidis FHbp, a virulence factor critical for surviving of the bacteria in the human host. FHbp protein is a bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH). Neisseria meningitidis is the leading global cause of meningitis and sepsis.
Application Notes: This antibody exhibited high affinity and specificity in the enzyme-linked immunosorbent assay (ELISA) and the whole bacterial cell enzyme-linked immunosorbent assay (Bact-ELISA). The binding affinity of the scFv version of this antibody for fHbp was reported to be Kd= 7.03 nM (PMID: 29737965).
Antibody first published in:
Yarian et al. Identification and characterization of a novel single-chain variable fragment (scFv) antibody against Neisseria meningitidis factor H-binding protein (fHbp) J Med Microbiol. 2018 Jun;67(6):820-827. PMID:29737965
Note on publication:
Describes the isolation and characterization of an scFv antibody against fHbp protein of N meningitidis.