Anti-FAP [73.3]

Description:

Recombinant monoclonal antibody to FAP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 73.3.

Clone Name:

73.3

Published Application(s):

in vitro, in vivo, IP, WB, FC

Published Species Reactivity:

Mouse

Products based on this clone:

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Ab03229-23.0 Anti-FAP [73.3]
Mouse
Rabbit IgG
Purified
In Stock

Ab03229-1.1 Anti-FAP [73.3]
Mouse
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: P97321

Alternative Name(s) of Target: Fibroblast activation protein; Fibroblast activation protein-α; Prolyl endopeptidase FAP; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha; FAPalpha1; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme;Serine integral membrane protease; SIMP; Surface-expressed protease; Seprase
Immunogen: The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells.

Specificity: The antibody is specific for fibroblast activation protein (FAP). FAP is a membrane protease that is highly expressed by cancer-associated fibroblasts (CAFs).

Application Notes: A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3ζ and 4–1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35–50% following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014; PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019; PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016; PMID: 26663085).

Antibody first published in: Wang et al. Targeting fibroblast activation protein in tumor stroma with chimeric antigen receptor T cells can inhibit tumor growth and augment host immunity without severe toxicity Cancer Immunol Res. 2014 Feb;2(2):154-66. PMID:24778279
Note on publication: The paper describes the generation of the antibody and the generation of the anti-muFAP-CAR construct.

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