United Kingdom (UK)
Recombinant monoclonal antibody to Fc. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody TP897.
Alternative Name(s) of Target: fragment crystallizable region; Fc region
Immunogen: This antibody was raised by immunising alpacas with 1.0 mg polyclonal rabbit IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Specificity: This antibody recognises Fc fragment of the rabbit IgG.
Application Notes: Anti-IgG nanobodies can often perform more efficiently than polyclonal secondary antibodies in various experimental assays. For instance, this nanobody conjugated with a maleimide-activated HRP was used to detect rabbit IgG via ECL Western blot and it considerably outperformed a polyclonal secondary antibody–HRP conjugate (Pleiner et al., 2018). Similarly, when conjugated with the infrared fluorophore IRDye 800, it was very efficient in detecting nucleoporins (Nups) in a Xenopus laevis egg extract via Western blot (Pleiner et al., 2018). TP897 antibody can be used for the purification of antigens or antigen complexes previously bound to a primary antibody, as it successfully isolated polyclonal rabbit IgG from serum and then released it under physiological conditions using SUMOStar protease cleavage (Pleiner et al., 2018). This nanobody is also recommended for the immunofluorescence detection of both monoclonal and polyclonal primary rabbit antibodies (Pleiner et al., 2018).
Antibody first published in:
Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID:29263082
Note on publication:
The article describes the generation and characterisation of this nanobody.