United Kingdom (UK)
Recombinant monoclonal antibody to FMG-1B. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 8.
UniProt Accession Number of Target Protein: Q84X68
Alternative Name(s) of Target: Flagella membrane glycoprotein 1B
Immunogen: The original mouse version of this antibody was raised by immunizing mice with flagella from C.reinhardtii.
Specificity: This antibody is specific for a carbohydrate epitope of Flagellar membrane glycoprotein 1B/ FMG-1B. FMG-1B is a protein part of the flagella of Chlamydomonas.
Application Notes: To study the distribution of the epitope to which this antibody binds. An ELISA was preformed on purified flagella from C.reinhardtii using the mouse version of this antibody (Bloodgood et al, 1986; pmid:3009491). Further to determine the unperturbed distribution of the mouse version of this antibody immunfluorescence was preformed on flagella from c.reinhardtii (Bloodgood et al, 1986; pmid:3009491). To determine the transport of IFT trains and the interaction of FMG proteins with this. The mouse version of this antibody was used for to cover beads which were subsequently used to monitor IFT trains (Shih et al, 2013; pmid:23795295).
Antibody first published in:
Bloodgood et al. Redistribution and shedding of flagellar membrane glycoproteins visualized using an anti-carbohydrate monoclonal antibody and concanavalin A J Cell Biol. 1986 May;102(5):1797-812. PMID:3009491
Note on publication:
This antibody was generated and charatarized.