United Kingdom (UK)
Recombinant monoclonal antibody to Folate receptor beta. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma CL10.
UniProt Accession Number of Target Protein: Q05685
Alternative Name(s) of Target: FRβ; Folate receptor 2; Folate-binding protein 2.
Immunogen: FRβ-transfected RBL2H3 cells.
Application Notes: The antibody binds specifically to FRβ, a member of the folate receptor family. The receptor binds with high affinity to folic acid and folic acid derivatives, and transports 5-methyltetrahydrofolate into the cell. The receptor is expressed on the surface of tumour associated macrophages (TAMs) that promote growth and metastasis of tumours such as gliomas and colon, breast and lung carcinomas. Research has shown FRβ is only expressed on activated macrophages, and thereby a monoclonal antibody that targets the receptor can be useful in the diagnosis of the cancer. Furthermore, the antibody may be conjugated with a toxin, delivering the drug only to TAMs and not to healthy macrophages.
Antibody first published in:
Nagai T, Tanaka M, Tsuneyoshi Y, Xu B, Michie SA, Hasui K, Hirano H, Arita K, Matsuyama T. Targeting tumor-associated macrophages in an experimental glioma model with a recombinant immunotoxin to folate receptor beta. Cancer Immunol Immunother. 2009 Oct;58(10):1577-86. PMID:19238383
Note on publication:
Describes the expression of folate receptor beta on macrophages in glioblastomas, and the production of an antibody against the receptor which can reduce tumour growth.
Western Blot using anti-folate receptor antibody Ab00498-1.1 Mouse heart (lane 1), mouse lung (lane 2), mouse pancreas (lane 3), mouse testis (lane 4) and rat ovary (lane 5)samples were resolved on a 12% SDS PAGE gel and blots probed with Ab00498-1.1 at 1.5 µg/ml before being detected by a secondary antibody. Bands correspondig to the expected 29 kDa folate receptor protein as well as dimers thereof were detected.