United Kingdom (UK)
Recombinant monoclonal antibody to Ft OAg. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N203 .
UniProt Accession Number of Target Protein: A0A0B6E216
Alternative Name(s) of Target: O-antigen of Francisella tularensis; O-antigen ligase family protein
Immunogen: The original antibody was generated by intradermal immunization of BALB/cJ mice with sub-lethal dose of Francisella tularensis holarctica strain LVS followed by intraperitoneal booster immunization with an outer membrane- or capsule-enriched LVS preparation.
Specificity: This antibody binds the internal O-antigen (OAg) of the gram-negative bacterium Francisella tularensis (Ft).
Application Notes: This antibody is recommended for the detection of the O anitgen of F. tularensis through ELISA. It is reported to react with high molecular weight F. tularensis lipopolysaccharide chains in a western bloy assay. In a mouse model of respiratory tularemia with the highly virulent Ft type A strain SchuS4, this antibody reduced blood bacterial burden with potencies that mirror its antigen-binding strength. N203 prolonged survival in a dose dependent manner. This clone is a less potent binder than Ab52 (PMID: 25171003).
Antibody first published in:
Lu et al. Functional and structural characterization of Francisella tularensis O-antigen antibodies at the low end of antigen reactivity. Monoclon Antib Immunodiagn Immunother. (2014); 33(4):235-45. PMID:25171003
Note on publication:
Describes the generation and characterization of this antibody.