United Kingdom (UK)
Recombinant monoclonal antibody to G. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HENV-26.
UniProt Accession Number of Target Protein: Q9IH62; F4YH71; H6V875; O89343
Alternative Name(s) of Target: RBP; Glycoprotein; Glycoprotein G; Attachment glycoprotein; Receptor binding protein;
Immunogen: The original antibody was isolated from an immune human individual.
Specificity: The antibody is specific for RBP of HeV and NiV. The antibody epitope overlaps with the receptor binding sites of RBP.
Application Notes: The specificity of the original format of the antibody for recombinant RBP head domain proteins from HeV, NiVM, or NiVB was confirmed by ELISA analysis (EC50 = 0.14,0.09 and 0.07 ug/mL respectively). The antibody could neutralize HeV, NiVB and NiVM in In vitro neutralization assays (IC50= 0.07, 0.03 and 0.04 ug/mL respectively). The antibody could bind to the full-length RBPs by flow cytometry. The EC50 values for binding of the antibody ranged from 325 to 343 ng/mL for binding to HeV, NiVM, or NiVB. The kinetics of binding of the antibody to RBPs was measured on a biosensor to determine affinity (KD= 2.9, 2.2, or 1.0 nM for HeV, NiVM, or NiVB, respectively). The crystal structures of Fab fragment of the antibody in complex with both HeV-RBP and NiV-RBP were determined. The antibody protected ferrets in lethal models of infection with NiV (Dong et al., 2020; PMID: 33306954).
Antibody first published in:
Dong et al. Potent Henipavirus Neutralization by Antibodies Recognizing Diverse Sites on Hendra and Nipah Virus Receptor Binding Protein Cell. 2020 Dec 10;183(6):1536-1550.e17. doi: 10.1016/j.cell.2020.11.023. PMID:33306954
Note on publication:
The original paper describes the generation and characterization of the antibody.